Case Studies

Mar 20, 2020

  1. Zongjie Wang
  2. Mark Gagliardi
  3. Reza M. Mohamad
  4. Sharif U. Ahmed
  5. Mahmoud Labib
  6. Libing Zhang
  7. Sandra Popescu
  8. Yuxiao Zhou
  9. Edward H. Sargent
  10. Gordon M. Keller and 
  11. Shana O. Kelley

Abstract

The ability to detect rare human pluripotent stem cells (hPSCs) in differentiated populations is critical for safeguarding the clinical translation of cell therapy, as these undifferentiated cells have the capacity to form teratomas in vivo. The detection of hPSCs must be performed using an approach compatible with traceable manufacturing of therapeutic cell products. Here, we report a novel microfluidic approach, stem cell quantitative cytometry (SCQC), for the quantification of rare hPSCs in hPSC-derived cardiomyocyte (CM) populations.  Read More

Aug 12, 2019

S.R.Bazaz, N.Kashaninejad, S.Azadi, K.Patel, M.Asadnia, M.E.Warkiani

Abstract

Polydimethylsiloxane (PDMS) is a long‐standing material of significant interest in microfluidics due to its unique features. As such, rapid prototyping of PDMS‐based microchannels is of great interest. The most prevalent and conventional method for fabrication of PDMS‐based microchips relies on softlithography, the main drawback of which is the preparation of a master mold, which is costly and time‐consuming. To prevent the attachment of PDMS to the master mold, silanization is necessary, which can be detrimental for cellular studies. Read More

May 26, 2019

H.Sorotsky, M.Aparanthi, D.Z.Wang, F.McFadden, S.N.Popescu, R.M.Mohamadi, M.Pereira, J.Weiss, D.Patel, S.Majeed, M.Cabanero, A.G.Sacher, P.A.Bradbury, N.B.Leighl, F.A.Shepherd, M.S.Tsao, G.Lui, S.O.Kelly, B.H.Lok

Abstract

Background: Tumor heterogeneity and evolution of SCLC is poorly defined. Serial longitudinal interrogation of tumor heterogeneity from CTCs detected in peripheral blood patient (pt) samples is a potential strategy to address this gap in knowledge. Read More

Lab on a Chip, 2016

E.M. Hamad , S.E.R. Bilatto, N.Y. Adly,  D.S. Correa, B.Wolfrum, M.J. Schoning, A.Offenhausser and A.Yakushenko

Abstract

Bonding of polymer-based microfluidics to polymer substrates still poses a challenge for Lab-On-a-Chip applications. Especially, when sensing elements are incorporated, patterned deposition of adhesives with curing at ambient conditions is required. Here, we demonstrate a fabrication method for fully printed microfluidic systems with sensing elements using inkjet and stereolithographic 3D-printing. Read More