CADWorks3D empowers Cellular Analytics from concept to commercialization.

Mar 20, 2020

  1. Zongjie Wang
  2. Mark Gagliardi
  3. Reza M. Mohamad
  4. Sharif U. Ahmed
  5. Mahmoud Labib
  6. Libing Zhang
  7. Sandra Popescu
  8. Yuxiao Zhou
  9. Edward H. Sargent
  10. Gordon M. Keller and 
  11. Shana O. Kelley

Abstract

The ability to detect rare human pluripotent stem cells (hPSCs) in differentiated populations is critical for safeguarding the clinical translation of cell therapy, as these undifferentiated cells have the capacity to form teratomas in vivo. The detection of hPSCs must be performed using an approach compatible with traceable manufacturing of therapeutic cell products. Here, we report a novel microfluidic approach, stem cell quantitative cytometry (SCQC), for the quantification of rare hPSCs in hPSC-derived cardiomyocyte (CM) populations.  Read More

Apr 2, 2018

Mahmoud Labib, Reza M. Mohamadi, Mahla Poudineh, Sharif U. Ahmed, Ivaylo Ivanov, Ching-Lung Huang, Maral Moosavi, Edward H. Sargent & Shana O. Kelley

Abstract

Cell-to-cell variation in gene expression creates a need for techniques that can characterize expression at the level of individual cells. This is particularly true for rare circulating tumour cells, in which subtyping and drug resistance are of intense interest. Here we describe a method for cell analysis—single-cell mRNA cytometry—that enables the isolation of rare cells from whole blood as a function of target mRNA sequencesRead More

Feb 06, 2018

Mahla Poudineh, Edward H. Sargent, Klaus Pantel & Shana O. Kelley

Abstract

During cancer progression, many tumours shed circulating tumour cells (CTCs) and other biomarkers into the bloodstream. The analysis of CTCs offers the prospect of collecting a liquid biopsy from a patient’s blood to predict and monitor therapeutic responses and tumour recurrence. In this Review, we discuss progress towards the isolation and recovery of bulk CTCs from whole blood samples for the identification of cells with high metastatic potential. Read More

Nov 21, 2016

Mahla Poudineh, Peter M. Aldridge, Sharif Ahmed, Brenda J. Green, Leyla Kermanshah, Vivian Nguyen, Carmen Tu, Reza M. Mohamadi, Robert K. Nam, Aaron Hansen, Srikala S. Sridhar, Antonio Finelli, Neil E. Fleshner, Anthony M. Joshua5, Edward H. Sargent and Shana O. Kelley

Abstract

Profiling the heterogeneous phenotypes of rare circulating tumour cells (CTCs) in whole blood is critical to unravelling the complex and dynamic properties of these potential clinical markers. This task is challenging because these cells are present at parts per billion levels among normal blood cells. Here we report a new nanoparticle-enabled method for CTC characterization, called magnetic ranking cytometry, which profiles CTCs on the basis of their surface expression phenotype. We achieve this using a microfluidic chip that successfully processes whole blood samples.  Read More